Characterization of the Blowfly Maggot (Lucilia robineau) Excretion/Saliva Extract.

Abstract

Maggots of the blowfly have been used for the treatment of antibiotics resistant wounds. The total protein concentration, isolation and characterization of biologically active proteins and peptides from Maggot saliva were determined by Bradford and SDS-PAGE gel electrophoresis. The total protein concentration was 430.51 mg/ml. SDS-PAGE gel electrophoresis of Maggot Excretion/Secretion revealed the presence of 5 proteins and peptides ranging from 17KDa to 121KDa. These results are obtained side by side with a proteins and peptides standard reference. The bands of proteins isolated were Beta galactosidase 121 KDa, Glycosylated bovin serum albumin 93 KDa, Ovalbumin 40KDa, Cardonic anhydrase 36 KDa, Beta lactoglobin 23 KDa and Lysozyme 17KDa