Haematopoetic Effect of Methanol Extract of Nigerian Honey Bee (Apis mellifera) Propolis in mice

Abstract

Objective: To investigate the haematopoetic effect of methanol extract of Nigerian honey bee propolis in mice. Methods: Fifteen white Albino mice were grouped into 3(A-C) of 5 animals each. Group A mice serve as control group, while groups B and C received 300 and 600 mL/kg of honey bee propolis respectively, for 21 days. The haematological parameters were determined using the automated haematologic analyzer Sysmex kx21, (product of Sysmex Corporation, Japan) using standard techniques. The data were analyzed using ANOVA and the level of significance was at P < 0.05. Acute oral toxicity study was conducted to determine it's safety on acute exposure. Results; Results showed significant (P < 0.05) dose dependent elevations in platelate count, mean platelate volume, plateletecrit, platelete distribution weight, white blood cells, granulocyte count, lymphocytes and mid cell total count. The extract however produce no significantly (P > 0.05) alteration to the erythrocytic indices like red blood cells, haematocrite, haemoglobin, mean corpuscular haemoglobin, mean corpuscular volume and red cell distribution width, but increase mean corpuscular haemoglobin concentration in dose related fashion. Acute oral toxicity showed the extract to be relatively safe at a high dose on acute exposure. However, 21-days of treatment with the extract do neither increase nor decrease the body weight of the mice. Objective: To investigate the haematopoetic effect of methanol extract of Nigerian honey bee propolis in mice. Methods: Fifteen white Albino mice were grouped into 3(A-C) of 5 animals each. Group A mice serve as control group, while groups B and C received 300 and 600 mL/kg of honey bee propolis respectively, for 21 days. The haematological parameters were determined using the automated haematologic analyzer Sysmex kx21, (product of Sysmex Corporation, Japan) using standard techniques. The data were analyzed using ANOVA and the level of significance was at P < 0.05. Acute oral toxicity study was conducted to determine it's safety on acute exposure. Results; Results showed significant (P < 0.05) dose dependent elevations in platelate count, mean platelate volume, plateletecrit, platelete distribution weight, white blood cells, granulocyte count, lymphocytes and mid cell total count. The extract however produce no significantly (P > 0.05) alteration to the erythrocytic indices like red blood cells, haematocrite, haemoglobin, mean corpuscular haemoglobin, mean corpuscular volume and red cell distribution width, but increase mean corpuscular haemoglobin concentration in dose related fashion. Acute oral toxicity showed the extract to be relatively safe at a high dose on acute exposure. However, 21-days of treatment with the extract do neither increase nor decrease the body weight of the mice. Conclusions: Administration of methanol extract of Nigerian honey bee propolis in mice at the doses investigated has brought about leucopoietic and thrombopoietic changes without any significant effect on red blood cells and factors that relate to it, except for the mean corpuscular haemoglobin concentration Conclusions: Administration of methanol extract of Nigerian honey bee propolis in mice at the doses investigated has brought about leucopoietic and thrombopoietic changes without any significant effect on red blood cells and factors that relate to it, except for the mean corpuscular haemoglobin concentration