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DC Field | Value | Language |
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dc.contributor.author | Oyedum, Uche Mary | - |
dc.contributor.author | Kuta, Faruk Adamu | - |
dc.contributor.author | Garba, Solomon A | - |
dc.contributor.author | Enejiyon, Sherifat Ozavize | - |
dc.date.accessioned | 2024-05-06T12:56:19Z | - |
dc.date.available | 2024-05-06T12:56:19Z | - |
dc.date.issued | 2021-09-27 | - |
dc.identifier.citation | Oyedum, U. M., Kuta, F. A., Garba, S. A., & Enejiyon, S. O. (2021). Comparative analysis of the phytochemical and antibacterial activity of the root extracts of Euphorbia heterophylla and Vitellaria paradoxa. African journal of clinical and Experimental Microbiology, 22 (4), 504 – 514. | en_US |
dc.identifier.issn | eISSN: 1595-689X | - |
dc.identifier.uri | http://repository.futminna.edu.ng:8080/jspui/handle/123456789/28008 | - |
dc.description.abstract | Background: Over time, herbal plants and their various components have been major sources of therapeutic medicine for man. A comparative study was carried out to determine the phytochemical components and antibacterial activities of the different crude extracts of Euphorbia heterophylla and Vitellaria paradoxa roots on four enteric bacteria; Salmonella typhi, Shigella flexneri, Escherichia coli and Proteus vulgaris. Methodology: Root samples of E. heterophylla and V. paradoxa were collected, washed, air dried and processed to fine powder in the microbiology laboratory of Federal University of Technology, Minna, Nigeria. Crude extract of the root samples was done by the cold maceration technique using four solvents (chloroform, methanol, petroleum ether and water). Phytochemical analysis of the extracts was done using previously described technique, and in vitro antibacterial activities of different concentrations of the extracts (50-200 mg/ml) and a standard antibiotic (ciprofloxacin) were tested on four enteric bacteria (S. typhi, S. flexneri, E. coli, P. vulgaris) by the agar diffusion test. In vivo antibacterial activities of the two plants were also tested by daily oral administration of 2000 mg/kg bodyweight (for 7 days) of each extract on inbred mice infected through intraperitoneal inoculation of an infective dose of each of the four enteric bacteria. Data were computed as mean ± standard error and analysed by the Statistical Analysis System (SAS) version 9.4. Associations between variables were determined using analysis of variance (ANOVA), with p < 0.05 considered as significant value. Results: Phytochemical analysis of the crude extracts of both plants revealed the presence of cardiac glycosides, saponins, alkaloids, flavonoids, and tannins but V. paradoxa contain more carbohydrates and starch, and less phlobatannins, compared to E. heterophylla. In vitro assay showed dose-dependent antibacterial activity of the methanol, aqueous and chloroform (but not petroleum ether) extracts of the two plant roots. The in vitro antibacterial activities of the different extracts of V. paradoxica extracts were significantly higher (higher mean diameters of inhibition zones) than those of E. heterophylla (p<0.05), and methanol extracts gave the highest antibacterial effects. However, the root extract of E. heterophylla gave a higher antibacterial activity with the in vivo assay on inbred mice than V. paradoxa, and methanol extracts of the two plant extracts gave the highest in vivo activity, followed by aqueous extract and least activity was obtained with the chloroform extract. Conclusion: Crude extracts of E. heterophylla and V. paradoxa roots produce antibacterial activity against enteric Gram-negative bacteria pathogens involved in diarrhoea illnesses. Further researches should be directed towards isolation and characterization of the active compounds in the crude extracts. | en_US |
dc.language.iso | en | en_US |
dc.publisher | African Journal of Clinical and Experimental Microbiology | en_US |
dc.relation.ispartofseries | 22;4 | - |
dc.subject | Phytochemicals | en_US |
dc.subject | In vitro | en_US |
dc.subject | In vivo | en_US |
dc.subject | Euphorbia heterophylla | en_US |
dc.subject | Vitellaria paradoxa | en_US |
dc.subject | Enteric bacteria | en_US |
dc.title | Comparative analysis of the phytochemical and antibacterial activity of the root extracts of Euphorbia heterophylla and Vitellaria paradoxa | en_US |
dc.type | Article | en_US |
Appears in Collections: | Microbiology |
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Comparative analysis of the phytochemical and antibacterial activity of the root extracts of Euphorbia heterophyllai and Vitellaria paradoxa.pdf | 879.86 kB | Adobe PDF | View/Open |
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