Evaluation of Locally Sourced Raw Materials for use as A Microbiological Media

Abstract

Local prepared nutrient media made from guinea corn and maize were used to culture bacteria such Staphylococcus aureus, Klebsiellasp, Salmonella typhi, Escherichia coli, and Pseudomonas aeruginosa and compared with the growth on commercial nutrient agar and broth. Bacteria growth was higher in the commercial nutrient agar and broth but with little significant difference. Locally prepared potato dextrose agar was also used to culture fungal isolates such as Aspergillusniger, Aspergillusfumigatus, Aspergillusflavus, Cephalosporiumsp, and Mucorsp and compared with commercial made potato dextrose agar using mycelia extension technique. Fungal growth was higher in commercially prepared potato dextrose agar with little difference. The results of these studies suggest that these locally sourced materials could be used for media preparation in place of commercially produced media. Key words: Microbiological Media, Locally prepared Nutrient M