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http://repository.futminna.edu.ng:8080/jspui/handle/123456789/16200| Title: | EFFECT OF SURFACTANT INCLUSIONS ON THE YIELD AND CHARACTERISTICS OF PROTEASE FROM BACILLUS SUBTILIS |
| Authors: | CHIDI EVANS, EGWIM Abdulkadir, Abdullahi |
| Keywords: | surfactant, protease, yield, characteristics, Bacillus subtilis. |
| Issue Date: | 2012 |
| Publisher: | ROMANIAN ACADEMY OF SCIENCES |
| Citation: | Egwim C. Evans and A. Abdullahi Proc. Rom. Acad., Series B, 2012, |
| Abstract: | In this study, the effect of surfactants (Tween-80 and Acetonitrile) inclusions in the culture medium of Bacillus subtilis on the yield and characteristics of extracellular protease was investigated. The results showed that addition of Tween-80 increased the enzyme yield by 5 folds and thermal stability from 50oC to 60oC. Tween-80 did not affect the optimum pH, KM and VMax values as well as the enzyme efficiency. Acetonitrile shifts the optimum pH from 8 to 7 and reduced the affinity of the enzyme for casein. The selection of appropriate surfactants for Bacillus subtilis culture is required for optimum protease production. |
| URI: | http://repository.futminna.edu.ng:8080/jspui/handle/123456789/16200 |
| Appears in Collections: | Biochemistry |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| art02Evans.pdf | 249.99 kB | Adobe PDF | View/Open |
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