Effects of single and mixed virus infections on the percent germination of some cowpea (Vigna unguiculata (L.) Walp) cultivars.

Abstract

A field trial was carried out to assess the response of twenty-five cultivars of cowpea to single and mixed infections with Blackeye cowpea mosaic virus (BlCMV) and Cowpea mottle virus (CPMoV) on seed quality. The field trial was conducted at the Teaching and Research Farm of the Faculty of Agriculture, Ahmadu Bello University (ABU), Zaria, Mokwa Station (090211ʹN and 50135ʹE, 201 m above sea level) situated in the Southern Guinea Savannah agro - ecological zone of Nigeria. The seed viability test was determined at the Crop Production Laboratory, Department of Crop Production, Federal University of Technology, Minna, Nigeria. Four independent trials were conducted simultaneously, for single and mixed infections. The field was cleared, ploughed, harrowed and ridged at 0.75 m apart then marked out into plots and replications. The trial was a randomized complete block design (RCBD) replicated three times giving a total land area of 900 m2. Three cowpea seeds of each cultivar were sown after dressing with Apron – star (methylthiuram + metalaxyl + carboxin) at the of rate 3.0 kg seed per 10 g of the chemical. Seeds were sown at an intra and inter– row spacing of 0.30 × 0.75 m along the ridges and later thinned to two per stand at 2 weeks after sowing (WAS). For the single virus infection, seedlings of the twenty five cultivars were inoculated at 10 days after sowing (DAS) while for the mixed virus infections,seedlings were inoculated at 10 and 17 DAS. The results of the experiment revealed that all cultivars were susceptible to single and mixed infections of the two viruses but to seemingly different extents. The viability of seeds from single infection with CPMoV was slightly reduced in some instances, but, even when seeds viability was not much affected, test of accelerated ageing for four weeks indicated that seed vigour was seriously impaired as compared to the other three virus treatments.