Experimental Invitro Treatment Of Coccidial OrganismsUsing Ethanolic Extract of Ripe Pawpaw Seed (Carica papaya) on Broiler Chickens

Abstract

Coccidiosis is one of the most expensive and common disease affecting poultry production in the tropics. Therefore, the need to create an effective organic measure to prevent the great loss is paramount. The organic anticoccidial effect was carried out using ripe pawpaw seed extract. Ripe pawpaw seeds were harvested, washed, air dried and grinded. 100g of powder was macerated in 1.5litres of ethanol and stirred at 3 hours interval daily for 72 hours and filtered using whatman paper No3. The filterate was concentrated by evaporating the solvent at 75 degree Celsius using rotatory evaporator (Buchi R-200) to obtain the extract. The extract was dissolved with Dimethyl sulphoxide (DMSO) and prepared at different concentration of 20, 30, 40 and 50mg/ml. the diluted extract was transferred into petri-dishes and placed equal amount of oocysts (2ml) was also added then, incubated at 28-30 degree Celsius for 24-30 hours. The sporozoites were counted using molasses counting chamber. The number of non-viable and viable sporozoites were estimated by counting the number of sporozoites in a total of 100 oocysts. The results obtained showed 52.9%, 50.7%, 33.3% and 24.6% of recovered sporozoites for 20, 30, 40 and 50mg/ml concentration respectively. The result showed the highest sporozoites inhibition was observed at concentration 50mg/ml at the rate of 24.6%. Therefore, it has shown that ripe pawpaw seed ethanolic extract can serve as an alternative to synthetic anticoccidial drugs and encouraged for use as it is cheaper and easily obtained.