EXPERIMENTAL INVITRO TREATMENT OF COCCIDIAL ORGANISMS USING ETHANOLIC EXTRACT OF RIPE PAWPAW SEED (CARICA PAPAYA) ON BROILER CHICKENS

Abstract

Coccidiosis is one of the most expensive and common diseases affecting poultry production in the tropics. Therefore, the need to create an effective organic measure to prevent the great loss is paramount. The organic anticoccidial effect was carried out using ripe pawpaw seed extract. Ripe pawpaw seeds were harvested, washed, air-dried and grinded. 100g of the powder was macerated in 1.5litres of ethanol and stirred at 3hours interval daily for 72hours and then filtered using whatman paper No 3. The filtrate was concentrated by evaporating the solvent at 750c using a rotatory eviporator (Buchi R-200) to obtain the extract. The extract was dissolved with Dimethyl sulphoxide (DMSO) and prepared at different concentration of 20, 30, 40, and 50mg/ml. The diluted extract was transferred into petric-dishes and placed equal amount of oocysts (2ml) was also added then, incubated at 28 – 300c for 24 – 30hours. The sporozoites were counted using molasses counting chamber. The number of non-viable and viable sporozoites were estimated by counting the number of sporozoites in a total of 100 oocysts. The results obtained showed 52.9%, 50.7%, 33.3% and 24.6% of recovered sporozoites for 20, 30, 40 and 50mg/ml concentration respectively. The result showed that the highest sporozoites inhibition was observed at concentration 50mg/ml at the rate of 24.6%. Therefore, it has shown that ripe pawpaw seed ethanolic extract can serve as an alternative to synthetic anticoccidial drugs and encouraged for use as it is cheaper and easily obtained.