Isolation and characterization of di- (1-hexen-5-yl) phthalate and monoethyl phthalate from the ethyl acetate extract of unripe fruits of Nauclea latifolia

Abstract

The aim of this study is to purify, identify and characterize some of the phytoconstituents in the ethyl acetate extract of the unripe fruits of Nauclea latifolia (Family Rubiaceae). the extract was subjected to a series of flash column chromatography, followed by purification with sephadex LH-20 to afford two phthalate derivatives that were subjected to physical, chemical and spectra identification by IR, UV, 1H-NMR, 13C-NMR, DEPT-135, GC-MS and literature search. The compounds were identified as Di-(1-hexen-5-yl) phthalate (34% yield). This is the first report of the isolation of these compounds from the unripe fruits of N. latifolia, although other phthalates have been reported