OIL DEGRADER FUNGI OF SPENT ENGINE OIL CONTAMINATED SOIL FROM SELECTED MECHANICWORKSHOPS IN MINNA, NIGERIA

Abstract

The aim of this study is to investigate the oil degrader fungi associated with degradation of spent oil Contaminated soil in five selected mechanic Workshops in Minna. Samples of the spent engine contaminated soils were collected from Shanchaga, Maikunkele, Shiroro,Tunga and Bosso mechanic workshops located in Minna Local Government Area. The fungi were isolated from the soils using dilution plate method salt Mineral in medium. All the fungi were identified based on macroscopic and microscopic features of the fruiting bodies and hyphal spores mass. A total of fifteen (15) fungal isolates belonging to eight genera were obtained from spent engine oil contaminated soil in all Sampled locations. Fungal species isolated were Aspergillus niger, Rhizopus stolonifer, Fusarium oxysporium, Aspergillus flavus, Penicillium notatum, Aspergillus fumigatus, Trichoderma harzianum Penicillium griseofulvum, Rhodotorula rubra Cunninghamella echinulata, Trichoderma viride Penicillium chrysogenum Mucor hiemalis Mucor Racemosus and Mucor plumbeus. Tunga soil sample had the highest fungal occurrence of isolates, followed by Bosso and Chanchaga while soil samples from Maikunkele and Shiroro had the least occurrences of fungi isolates. Aspergillus niger, Rhizopus stolonifera And Aspergillus flavus were the prominent isolates from all the sampled locations while Penicillium chrysogenum, Mucor hiemalis Cunninsghamella echinulata,Mucor plumbeus and Rhodotorula sp had least number of the occurrence from all locationsW.The presence of these three predominant fungalspecies in the spent engine 1J02 oil contaminated soils samples collected from the selected all mechanic workshops Minna within metropolis is a strong indication that these fungi can be use to oil restoring Contaminated environments through mycoremediation process.