Production and Partial characterization of ProteaseUsing Mango Seed Kernel as Carbon Source by Bacillus megaterium

Abstract

Proteases are the hydrolase enzymes that catalyze the hydrolysis of the peptide bonds in the primary structure of proteins and peptides. Mango seed Kernel was evaluated to check the possibility of potential utilization as a carbon source of protease production by bacillus megaterium. Protease assay was carried out using casein as substrate and activity extrapolated from standard tyrosine caliberation curve. The Km and Vmax of protease was extrapolated from double reciprocal plot. The studies show that the protease production by B. megaterium was higher using mango seed kernel as carbon source than in glucose. The production was optimal using mando seed kernel at concentration of 1.5g, ph 5 after 5 days of incubation. The characterization of protease by B. megaterium grown on mango seed kernel has optimal activity at pH 4, temperature of 60oC and at 1% casein concentration with high Vmax and low Km. It can be concluded that mango seed kernel can be used to replace the costly glucose in microbial enzyme production particularly protease