Browsing by Author "Okoye C.I."

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    Biochemical evaluation of mutant genotypes of Sesamum indicum for the development of improved varieties
    (Botanica Lithuanica, 2022-12-21) Audu M.A.I; Idris Y.; Muhammad L.M.; Falusi O.A.; Salihu I.A.; Okoye C.I.
    The nutritional attributes of sesame, Sesamum indicum, vary substantially and are primarily influenced by the plant’s genetic makeup. This investigation accessed the biochemical composition of sesame mutants from two generations. Seeds of eleven M3 lines were planted in a Randomized Complete Block Design (RCBD) to raise M4 lines, which were advanced to M5 generation. The seeds were harvested at maturity, and their biochemical compositions were quantitatively determined following standard procedures. Results revealed significant diffe rences (p < 0.05) in the biochemical composition of the mutants across the two generations. Exactly 27.27% of the mutants showed a substantial increase in oil composition over their parental varieties at the M4 generation. In comparison, 36.36% of the mutants had higher oil contents than their parental varieties at the M5 generation. The M5 mutants showed significantly (p < 0.05) higher oil content than the M4 mutants. Significant differences (p < 0.05) were observed in the protein content of M4 and M5 mutants, with M4 mutants having higher protein content. Significant differences (p < 0.05) were observed in fibre, carbohydrate, energy, and moisture contents of M4 and M5 mutants, with M5 mutants having significantly higher (p < 0.05) carbohydrate and energy con tents, while no significant difference (p > 0.05) was observed in ash content of M4 and M5 mutants. Results also reveal significant differences (p < 0.05) in anti-nutrients of M4 and M5 mutants with notable reductions in some anti-nutritional properties of some mutant lines. High and stable nutritional attributes are desirable traits in sesame breeding. The identified mutants with desirable traits are potential candidates that could be selected and developed as the first mutant varieties of sesame in Nigeria
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    Effect of fast neutron irradiation on agronomic traits of three tomato (Solanum lycopersicum) cultivars
    (NABDA Journal of Biotechnology Research, 2023-08-07) Okoye C.I.; Falusi O.A.; Aminu A.; Mohammed A.; Audu M.A.I
    Fast neutron irradiation (FNI) has been successfully utilized in the development of plant varieties with superior agronomic traits. This study thus, investigated the effect of different irradiation doses on some agronomic parameters of three selected tomato accessions (NG/MR/5/9/006, NG/AA/9/9/037 and NHGB/09/114). Exactly two hundred seeds of each accession were exposed to FNI obtained from an Americium-Beryllium source with a flux of 1.5×104 cm-2s-1 for 30, 60, 90 and 120 minutes, equivalent to 4 rad, 8 rad, 16 rad and 24 rad, respectively. The non-irradiated seeds served as control. The seeds were planted in experimental pots arranged in a Randomized Complete Block Design (RCBD) in three replicates. Results revealed significant differences (p < 0.05) in germination percentage with control having the significantly (p < 0.05) highest (90 %) in NG/MR/5/9/006 while NG/AA/9/9/037 and NHGB/09/114 revealed significantly (p < 0.05) higher germination percentage of 90 % and 83 %, respectively at 4 rad. No significant difference (p > 0.05) was observed in number of fruits per plant of NG/MR/5/9/006. A significant increase (p < .05) was observed in number of fruits per plant of NG/AA/9/9/037 and NHGB/09/114 at 4 rad (2.60 ± 0.60 and 4.00 ± 1.05, respectively) and 24 rad (2.60 ± 0.51 and 4.40 ± 1.12, respectively). The significantly highest weight of fruits and number of branches at fruiting were recorded at 90 minutes of exposure (16 rad) in all the accessions. NG/AA/9/9/037 had significantly higher number of seeds per fruit (107.33 ± 6.67) while no significant difference was observed in number of seeds per fruit. This study revealed significant enhancement in some growth and yield parameters of seeds exposed to fast neutron irradiation and demonstrates that fast neutron irradiation is an excellent tool for enhancing the efficiency of breeding Solanum lycopersicum and evolving higher yield variants through proper selection.